Mycobacterium tuberculosis complex: Drug susceptibility testing; First-line
| Test Name: | Mycobacterium tuberculosis complex: Drug susceptibility testing; First-line |
| Test Methodology: | Phenotypic drug susceptibility testing of Mycobacterium tuberculosis complex at critical concentrations by BACTEC MGIT 960 system. DST panel include isoniazid (INH) 0.1 µg/ml, rifampin (RIF) 1.0 µg/ml, ethambutol (EMB) 5.0 µg/ml, and pyrazinamide (PZA) 100 µg/ml. |
| Test to be Performed: | MTB MGIT Susceptibility |
| CPT: | 87188: Each drug |
| Reflex Testing Criteria: | None |
| Pre-Approval Required: | None, unless duplicate submission (e.g., samples from the same patient and same source collected within 3 months from prior collection date). |
| Accepted Specimens: | Pure culture of viable Mycobacterium tuberculosis complex on solid media. Slants (LJ/7H10) are the preferred culture media for submission. |
| Specimen Stability: | Room temperature: up to 4 weeks. Refrigerated (2 – 8°C): up to 6 months. |
| Storage/Transport Conditions: | Ambient temperature. Ensure isolate is secured with parafilm or tape. Ship using appropriate DOT/IATA requirements. Suspected and/or confirmed MTBC cultures should be shipped as Category A Infectious Substance. |
| Turnaround Time: | 17 days; preliminary report will be issued if resistance is detected and needs to be repeated for confirmatory purposes. |
| Reference Range: | Susceptible |
| Comments: | For questions regarding submissions, please contact the lab for further information. |
Public Health Laboratory Location
150 East Holt Boulevard
Ontario, CA 91761
Phone: (909) 458-9430
Fax: (909) 986-3590
Monday – Friday: 8 a.m. to 5 p.m.